Invitrogen™ Human LAG-3 (CD223) Fc Chimera Recombinant Protein

Product Code.	Quantity	Unit Size
17986261	500 μg	500µg
17965861	50 μg	50µg

Product Code. 17986261 Supplier Invitrogen™ Supplier No. RP88096

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (330 μL) be added to the vial to prepare a stock solution of 0.15 mg/mL. Concentration is measured by UV.

LAG-3 is a 70-kDa surface glycoprotein in the immunoglobulin superfamily, with structural homology to CD4. It binds to MHC class II molecules with higher affinity than CD4 and is involved in the negative regulation of T cell activation and homeostatic proliferation. LAG-3 is expressed on the surface of activated T cells, including regulatory T cells, and NK cells. Notably, CD8+ T cells express LAG-3 at significantly higher levels than CD4+ T cells. Additionally, the coexpression of LAG-3 and CD49b has been proposed as a marker for identifying Type 1 regulatory T cells (Tr1 cells) in both humans and mice. This highlights LAG-3s role in immune regulation and its potential as a target for therapeutic interventions in immune-related conditions.

Specifications

• Accession Number: P18627
• For Use With (Application): Binding Assay, Control, ELISA Standard
• Format: Lyophilized
• Formulation: PBS with 15% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3902
• Molecular Weight (g/mol): 72.58 kDa
• Name: Human LAG-3 (CD223) Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 500 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3lymphocyte activating 3; soluble LAG3
• Common Name: LAG-3 (CD223)
• Gene Symbol: LAG3
• Biological Activity: Immobilized LAG3 Protein, Human, Recombinant (ECD, mFc Tag) (at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human LAG3, amino acids Met1-Leu450 (Accession # NP_002277.4) with a C-terminal mouse IgG1 Fc region
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized LAG3 Protein, Human, Recombinant (ECD, mFc Tag) at 2 μg/mL (100 μL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE.