Invitrogen™ Human Kallikrein 14 ELISA Kit

Product Code. 15445053

Brand: Invitrogen™ EHKLK14

Description

ELISA

The Human Kallikrein 14 (Hu KLK14) ELISA quantitates Hu KLK14 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu KLK14. Principle of the method The Human KLK14 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Kallikreins are a subgroup of serine proteases and are implicated in carcinogenesis. A novel KLK gene, KLK12, is expressed in a variety of tissues including salivary gland, stomach, uterus, lung, thymus, prostate, colon, brain, thyroid, and trachea. It has applications as a novel cancer biomarker.

Specifications

• Accession Number: Q9P0G3
• Assay Range: 2.05 to 500 pg/mL
• Assay Sensitivity: 1.5 pg/mL
• Calibration Range: 1.5 pg/mL to 500 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: GCPR
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human KLK14. Other species not determined.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 43847
• Gene Symbol: KLK-L6
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Biomarker-Related Pathways
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: KLK-L6
• Target: Kallikrein 14
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins