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Gibco™ Human Keratinocyte Growth Supplement (HKGS)
Description
A sterile, concentrated (100X) solution intended for use with EpiLife™ medium or Medium 154 to culture human epidermal keratinocytes. HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), recombinant human insulin-like growth factor-I, hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5 mL bottle of HKGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes, and is the correct amount of supplement for a 500 mL bottle of medium.
When a 500 mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented medium are:
When a 500 mL bottle of medium is supplemented with HKGS, the final concentrations of the components in the supplemented medium are:
- Bovine pituitary extract (BPE), 0.2% v/v
- Recombinant human insulin-like growth factor-I, 0.01 μg/mL
- Hydrocortisone, 0.18μg/mL
- Bovine transferrin, 5μg/mL
- Human epidermal growth factor, 0.2 ng/mL
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -20°C). |
| Cell Type | Keratinocyte Cells |
| Concentration | 100 X |
| Form | Liquid |
| Product Type | Growth Supplement |
| Sterility | Sterile |
| For Use With (Application) | Mammalian Cell Culture, Primary Cell Culture |
| Product Line | Cascade Biologics |
| Quantity | 5 mL |
| Source | Human |
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For Research Use Only. Not for use in diagnostic procedures.
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