Invitrogen™ IL-21 Human ELISA Kit

Sandwich ELISA Kit

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-8/NAP-1
Biotin-Conjugate anti-human IL-8/NAP-1 polyclonal antibody
Streptavidin-HRP
Human IL-8/NAP-1 Standard, 100ng/mL
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Product Code	Brand	Quantity
15501567	Invitrogen™ BMS2043	96 Tests
15511567	Invitrogen™ BMS204-3TEN	10 x 96 Tests

Description

The Human Interleukin-8 (Neutrophil Activating Peptide-1) (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Specifications

• Accession Number: P10145
• Assay Sensitivity: 2.0 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
• Intraassay CV: 6.3%
• Label or Dye: HRP
• Sample Volume: Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Protein 3-10C, CXCL8, NAP-1, GCP-1
• Test Time: 1 hr. 20 min.

• Assay Range: 15.6-1,000 pg/mL
• Conjugate: Biotin
• Sample Type: Amniotic Fluid, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3576
• Interassay CV: 8.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Assay Buffer concentrate, SAV-HRP, SAV-HRP, Controls, Chromogen, Chromogen, Stop Solution
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: IL-21
• Total Assay Time: 3 hr. 10 min.

For Research Use Only