Thermo Scientific™ anti-Interleukin 6 Recombinant, Thermo Scientific™

Interleukin 6 Recombinant protein SIL6 from Thermo Scientific™ specifically detects Interleukin 6 in Not Applicable samples, and it is validated for Control, ELISA and WB

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ESS0005

HUMAN IL-6 SCREENING SET

UNSPSC: 41116010

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10761365

Quantity Price
1 £ 369.0 / 1 set
Estimated Shipment date
from Supplier 02-01-2017
Add to basket


Description and Specification

Specification

Assay Range 10.2 to 400pg/mL
Description Human IL-6 ELISA Reagent Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Antibodies and complete reagent set for ELISA
Kit Contents Human IL-6 Standard, 5 vials
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 5 plate kit
Sensitivity <1pg/mL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Sufficient For 5 x 96 microplate wells
Total Assay Time 3 hr.
Target Natural and recombinant human interleukin-6 (IL-6)

These human IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.2 to 400pg/mL

  • Sensitivity: < 1pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFα, IFNγ, IFNα, GM-CSF, or mouse IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).