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Thermo Scientific™ Human IL-5 ELISA Kits
Measure levels of human interleukin-5 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Brand: Thermo Scientific™ EHIL5
Description
Assay IL-5 (human interleukin-5) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-5 ELISA Kits.
This human IL-5 receptor ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble interleukin-5 (IL-5) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human interleukin-5 (IL-5)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 0 to 400pg/mL
Sensitivity: < 2pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, urine serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, TNFα, IFNγ, IFNα, GM-CSF, GROα, GROβ, or mouse IL-5
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifications
0 to 400pg/mL | |
Human IL-5 ELISA Kit | |
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine | |
96 microplate wells | |
Absorbance at 450nm minus absorbance at 550nm | |
1 Plate Kit | |
3 hr. |
<2pg/mL | |
Colorimetric ELISA (TMB substrate) with Plates | |
No significant cross-reaction with human IL-1a, IL-1ß, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, TNFa, IFNg, IFNa, GM-CSF, GROa, GROß, or mouse IL-5 | |
Biotinylated Antibody Reagent, 8mL, 30X Wash Buffer, 50mL, Anti-Human IL-5 Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-5 Standard, 2 vials, Standard Diluent, 12mL, Stop Solution, 13mL, Adhesive Plate Covers, 6 ea, Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL | |
Intra-assay CV <10%; inter-assay CV <10% | |
Natural and recombinant human interleukin-5 (IL-5) |