Thermo Scientific™ Human IL-5 ELISA Kits

Measure levels of human interleukin-5 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ EHIL5

Product Code. 11865954

Description

Assay IL-5 (human interleukin-5) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-5 ELISA Kits.

This human IL-5 receptor ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble interleukin-5 (IL-5) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-5 (IL-5)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 0 to 400pg/mL

• Sensitivity: < 2pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, urine serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, TNFα, IFNγ, IFNα, GM-CSF, GROα, GROβ, or mouse IL-5

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Assay Range: 0 to 400pg/mL
• Description: Human IL-5 ELISA Kit
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
• Sufficient For: 96 microplate wells
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 1 Plate Kit
• Total Assay Time: 3 hr.

• Assay Sensitivity: <2pg/mL
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with human IL-1a, IL-1ß, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, TNFa, IFNg, IFNa, GM-CSF, GROa, GROß, or mouse IL-5
• Kit Contents: Biotinylated Antibody Reagent, 8mL, 30X Wash Buffer, 50mL, Anti-Human IL-5 Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-5 Standard, 2 vials, Standard Diluent, 12mL, Stop Solution, 13mL, Adhesive Plate Covers, 6 ea, Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Target: Natural and recombinant human interleukin-5 (IL-5)