Invitrogen™ IL-18 Human Instant ELISA™ Kit

Product Code. 15541857

Brand: Invitrogen™ BMS267INST

Description

Includes

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-18, HRP-Conjugate (human IL-18 monoclonal antibody) and Sample Diluent, lyophilized
• Aluminum pouch(es) with a human IL-18 Standard curve (coloured)
• Wash Buffer Concentrate 20x

Instant ELISA Kit

The Human Interleukin 18 (Hu IL-18) ELISA quantitates Hu IL-18 in human serum, plasma, cell culture supernatants, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-18. Principle of the method The Human IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

• Accession Number: Q14116
• Assay Range: 78-5,000 pg/mL
• Assay Sensitivity: 9.2 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3606
• Gene Symbol: IGIF,IL-18,IL-1g,IL1F4
• Interassay CV: 8.1%
• Intraassay CV: 6.5%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL
• Storage Requirements: -20°C
• Synonym: Interleukin-18, Interferon-gamma-inducing factor
• Target: Human
• Test Time: 20 min.
• Total Assay Time: 3 hr. 10 min.

For Research Use Only