Invitrogen™ eBioscience™ Mouse M-CSF, Recombinant Protein

Mouse Recombinant Protein

Brand:  Invitrogen™ 14-8983-80

Product Code. 15547556

Description

Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization.

M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex.

Specifications

• Accession Number: NM_001113530
• For Use With (Application): Cytokine Bioassays
• Molecular Weight: 37.79kDa
• Purification Method: SDS-PAGE
• Quantity: 25μg
• Storage Requirements: Store at less than or equal to -70°C.
• Structural Form: Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530
• Gene Symbol: Macrophage colony stimulating factor, monocyte colony stimulating factor
• Product Type: Recombinant Protein
• Cross Reactivity: Mouse

• Concentration: 0.1mg/mL
• Formulation: Sterile liquid; phosphate buffered saline with 1% BSA, pH 7.2 0.22μm filtered.
• Name: M-CSF
• Purity: >98%
• Source: Insect Cells
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Biological Activity: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10⁵ Units/mg.
• Conjugate: Unlabeled
• Recombinant: Yes