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Mouse M-CSF, Recombinant Protein, eBioscience™

Recombinant Protein

Brand:  Affymetrix eBioscience 14-8983-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15547556

  • £88.79 / 25µg


EU Stock 10
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Description

Description

Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Description: Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Applications Reported: Recombinant mouse M-CSF is biologically active. Applications Tested: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Source: Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530. Bioactivity: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 37.79 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

P07141
Cytokine Bioassays
PBS with 1% BSA and no preservative; pH 7.2
37.79kDa
7.2
Affinity chromatography
Insect Cells
RUO
Recombinant
Mouse M-CSF
Control, Functional Assay
Recombinant Protein
Unlabeled
Insect cells
0.1mg/mL
Liquid
12977
Mouse M-CSF
SDS-PAGE
25μg
-80°C
Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Csf1
The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 105 Units/mg.
Mouse
Ms
Recombinant
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