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Mouse M-CSF, Recombinant Protein, eBioscience™
Recombinant Protein
Brand: Affymetrix eBioscience 14-8983-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description
Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Description: Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Applications Reported: Recombinant mouse M-CSF is biologically active. Applications Tested: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Source: Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530. Bioactivity: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10e5 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 37.79 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.Specifications
| P07141 | |
| Cytokine Bioassays | |
| PBS with 1% BSA and no preservative; pH 7.2 | |
| 37.79kDa | |
| 7.2 | |
| Affinity chromatography | |
| Insect Cells | |
| RUO | |
| Recombinant | |
| Mouse M-CSF | |
| Control, Functional Assay | |
| Recombinant Protein | |
| Unlabeled | |
| Insect cells |
| 0.1mg/mL | |
| Liquid | |
| 12977 | |
| Mouse M-CSF | |
| SDS-PAGE | |
| 25μg | |
| -80°C | |
| Insect expressed C-terminal His-tagged Lys33-Pro187, accession number NM_001113530 | |
| Less than 0.01ng/μg cytokine as determined by the LAL assay. | |
| Csf1 | |
| The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 105 Units/mg. | |
| Mouse | |
| Ms | |
| Recombinant |