eBioscience™ Human CD25, Recombinant Protein, Carrier-Free

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8702-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15558826

Quantity Price
1 £ 146.13 / 25µg
Estimated Shipment date
from Supplier 06-06-2017
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Description and Specification

Specification

Purity >98%
Recombinant Yes
Structural Form Insect cells (amino acids Glu22- Gln240; Accession #: NP_000408.1)
Molecular Weight 24.8kDa
Formulation Lyophilized from 1X PBS.
Source Insect Cells
For Use With (Application) Functional Assays
Storage Requirements Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Quantity 25μg
Name CD25
Conjugate Unlabeled
Biological Activity The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1ng/mL IL-2, is 0.5-1.5μg/mL.
Regulatory Status RUO
Product Type Recombinant Protein, Carrier-Free
Cross Reactivity Human
Purification Method SDS-PAGE and HPLC
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay

The human soluble IL-2 receptor alpha chain (sIL-2Ra) is generated by proteolytic cleavage of the cell-surface bound receptor on activated T cells. The IL-2Ra (also known as CD25) is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 with low affinity. However, association with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) results in formation of the high affinity IL-2R. CD25 plays a role in lymphocyte differentiation and activation/proliferation. sIL-2Ra has been detected in the serum of patients with autoimmune disease and leukemia. Reports have demonstrated that addition of sIL-2Ra can inhibit IL-2-mediated signaling and enhance Th17 responses.