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Thermo Scientific™ Pierce™ Human IL-10 ELISA Kits

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ EHIL102

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10443425

  • £457.00 / 1 set


EU Stock 10
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Specifications

Description

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

Intra-assay CV <10%; inter-assay CV <10%
Complete ELISA kit with coated 96-well plates
30X Wash Buffer, 2 x 50mL
2 x 96 microplate wells
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
50µL
3 hr.
<3pg/mL
Absorbance at 450nm minus absorbance at 550nm
15.4 to 600pg/mL
No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
Natural and recombinant human interleukin-10 (IL-10)
Human IL-10 ELISA Kit
2 plate kit