Gibco™ Human IL-1 beta Recombinant Protein

Product Code. 10415533

Brand: Gibco™ PHC0811

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized hIL-1 beta should be reconstituted in deionized water to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Storage: Lyophilized hIL-1 beta should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted hIL-1 beta at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Accession Number: P01584
• For Use With (Application): Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 3553
• Molecular Weight (g/mol): 17.5 kDa
• Name: Human IL-1 beta
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: 4°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: catabolin; cytokine; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1; IL1 B; IL-1 beta; IL-1 precursor; IL1B; IL-1B; IL1B1; IL1beta; IL-1beta; IL1-BETA; IL1F2; IL1β; ILN; Interleukin; interleukin 1 beta; Interleukin 1 beta precursor; interleukin 1, beta; interleukin 1, beta 1; interleukin 1-beta; interleukin*1*beta; Interleukin1 beta; interleukin-1 beta; interleukin-1 beta precursor; interleukin-1 beta precursor (AA -113 to 153); interleukin-1 beta proprotein; interleukin-1b; interleukin-1beta; interleukin-beta; LAF; lymphocyte proliferation-potentiating factor; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; prointerleukin-1 beta; pro-interleukin-1-beta
• Common Name: IL-1 beta
• Gene Symbol: IL1B
• Biological Activity: ED50 = 1.4 - 4 pg/mL; determined by the dose-dependent proliferation of murine D10S cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
• Content And Storage: 4°C
• Classification: Carrier-Free
• Activity: ED50 = 1.4 - 4 pg/mL; determined by the dose-dependent proliferation of murine D10S cells.
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Stem Cell Research, Immunology
• Product Line: Gibco
• Purity or Quality Grade: >95% by SDS-PAGE