Invitrogen™ Human IL-1 beta ELISA Kits

Product Code. 10713295

Brand: Invitrogen™ EH2IL1B

Description

Measure levels of human interleukin-1 beta (IL-1b) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Assay IL-1b (human interleukin-1 beta) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-1 beta ELISA Kits.

These human IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-1β antibody pairs and IL-1β standard only. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-1 beta (IL-1β)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 10.2 to 400pg/mL

• Sensitivity: ≤ 1pg/mL

• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 4 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human IL-1α, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, IFNα, IFNγ or mouse IL-1β

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

• Assay Range: 10.2 to 400pg/mL
• Assay Sensitivity: ≤ 1pg/mL
• Description: Human IL-1 beta ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Specificity: No significant cross-reaction with human IL-1a, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, IFNa, IFNg or mouse IL-1ß
• Sufficient For: 96 microplate wells
• Kit Contents: HRP Dilution Buffer, 14mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Biotinylated Antibody Reagent, 8mL, Anti-Human IL-1 beta Precoated 96-well Strip Plate, 1 ea, Lyophilized Recombinant Human IL-1 beta Standard, 2 vials, TMB Substrate Solution, 13mL, Streptavidin-HRP Concentrate, 75 μL, Stop Solution, 13mL, Standard Diluent, 12mL
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Quantity: 1 Plate Kit
• Sample Volume: 50 μL
• Target: Natural and recombinant human interleukin-1 beta (IL-1ß)
• Total Assay Time: 4 hr.