Invitrogen™ Human IL-1 beta ELISA Development Kit (TMB), PeproTech®

Product Code. 30088330

Brand: Invitrogen™ 900T95K

Description

ELISA

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Accession Number: P01584
• Assay Range: Lot-specific
• Assay Sensitivity: Lot-specific
• Conjugate: HRP
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3553
• Gene Symbol: IL1B
• Kit Contents: Capture Antibody, Detection Antibody, Protein Standard, HRP-conjugate
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: IL-1 beta
• Test Time: 1 hrs 45 mins
• Total Assay Time: 24 hrs 30 mins