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Pierce™ Human IL-1 alpha ELISA Kits

Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Brand:  Invitrogen EH2IL1A2

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10289803

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Description

Description

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-1 alpha (IL-1α)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 10.2 to 400pg/mL

  • Sensitivity: < 2pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

10.2 to 400pg/mL
Human IL-1 alpha ELISA Kit
Complete ELISA kit with coated 96-well plates
Absorbance at 450nm minus absorbance at 550nm
<2pg/mL
50µL
2 x 96 microplate wells
Natural and recombinant human interleukin-1 alpha (IL-1a)
30X Wash Buffer, 2 x 50mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
2 plate kit
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IFNa, IFNg, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, or mouse IL-1a. Human IL-1ra does not interfere with this ELISA
3 hr.
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