Invitrogen™ Human IL-1 alpha ELISA Kits

Product Code.	Quantity	unitSize
10289803	2 Plate Kit	1 set
10066023	1 Plate Kit	1 set
10711565	5 Plate Kit	1 set

Product Code. 10289803 Supplier Invitrogen™ Supplier No. EH2IL1A2

Description

Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Assay IL-1a (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-1 alpha ELISA Kits.

These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human interleukin-1 alpha (IL-1α)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 10.2 to 400pg/mL

• Sensitivity: < 2pg/mL

• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Assay Range: 10.2 to 400pg/mL
• Assay Sensitivity: <2pg/mL
• Description: Human IL-1 alpha ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
• Specificity: No significant cross-reaction with human IFNa, IFNg, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, or mouse IL-1a. Human IL-1ra does not interfere with this ELISA
• Sufficient For: 2 x 96 microplate wells
• Kit Contents: Streptavidin-HRP Concentrate, 2 x 75 μL, TMB Substrate Solution, 2 x 13mL, Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 2 ea, 30X Wash Buffer, 2 x 50mL, Adhesive Plate Covers, 12 ea, Biotinylated Antibody Reagent, 2 x 8mL, HRP Dilution Buffer, 2 x 14mL, Lyophilized Recombinant Human IL-1 alpha Standard, 4 vials, Standard Diluent, 2 x 12mL, Stop Solution, 2 x 13mL
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Quantity: 2 Plate Kit
• Sample Volume: 50 μL
• Target: Natural and recombinant human interleukin-1 alpha (IL-1a)
• Total Assay Time: 3 hr.