Invitrogen™ Human IgG1 (103 Cys/Ser) Fc Chimera Recombinant Protein

Product Code. 17184223

Brand: Invitrogen™ A42560

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). [UniProt].

Specifications

• Accession Number: P01857
• For Use With (Application): Control, ELISA Standard, Binding Assay, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3500
• Molecular Weight (g/mol): 26 kDa
• Name: Human IgG1 (103 Cys/Ser) Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 5 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: IgG1; IgG3; Igh-4; Ighg; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin heavy chain (gamma polypeptide); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); RGD1359539; VH7183
• Common Name: IGHG1
• Gene Symbol: IGHG1
• Biological Activity: 1. Measured by its ability to bind human CD16a-His in a functional ELISA. 2. Measured by its ability to bind human CD16a-AVI-His in a functional ELISA. 3.Measured by its ability to bind human CD16a-His in a functional ELISA.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: IgG1 Fc Tag
• Sequence: Human IgG1 Fc, amino acids Glu99-Lys330 (Accession # P01857-1) with a single mutation C103S
• Content And Storage: Store at -20 to -70 °C
• Activity: 1. Measured by its ability to bind human CD16a-His in a functional ELISA.<br><br>2. Measured by its ability to bind human CD16a-AVI-His in a functional ELISA.<br><br>3.Measured by its ability to bind human CD16a-His in a functional ELISA.
• Endotoxin Level: < 1 EU/μg
• Shipping Condition: Room Temperature
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Truncated
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Immunology, Inflammation
• Purity or Quality Grade: ≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.
• Protein: Fc region of human immunoglobulin G1