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STEMCELL Technologies Human IgE ELISA Kit

Product Code. 17158041
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Product Code. 17158041 Supplier STEMCELL Technologies Supplier No. 02032

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Description

For detection and measurement of human immunoglobulin E

  • The Human Immunoglobulin E (IgE) ELISA Kit is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants
  • IgE is found in the lungs, skin, and mucous membranes
  • IgE plays a role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI)
  • The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin
  • The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody
  • Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample

Specifications

Description B Cells; Hybridomas
Quantity 2 Plates
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