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Human IL-29 (IFN lambda 1) Carrier-Free, Invitrogen™

Product Code. 15538776
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Quantity:
20 μg
Unit Size:
20µg
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Product Code. 15538776

Brand: Invitrogen 34829864

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Recombinant Protein

IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity. Description: IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity. Applications Reported: This protein has been demonstrated to induce STAT2 phosphorylation. Applications Tested: Recombinant human IL-29 (IFN lambda 1) is biologically active and can induce STAT2 phosphorylation. Source: E. coli (amino acids Pro23-Thr178; accession # NP_742152.1). Bioactivity: This protein has been demonstrated to induce STAT2 phosphorylation. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 19.8 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of less than 0.5 - 1.0mg/mL. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q8IU54
For Use With (Application) Control, Functional Assay
Formulation PBS with no preservative
Gene ID (Entrez) 282618
Molecular Weight (g/mol) 19.8kDa
Name Human IL-29 (IFN lambda 1) Carrier-Free
Purification Method SDS-PAGE and HPLC
Quantity 20 μg
Source E. coli
Storage Requirements -20°C
Regulatory Status RUO
Structural Form E. coli (amino acids Pro23 - Thr178; Accession # NP_742152.1)
Endotoxin Concentration Less than 0.1ng/μg cytokine as determined by the LAL assay
Common Name Human IL-29 (IFN lambda 1) Carrier-Free
Gene Symbol IFNL1
Biological Activity This protein has been demonstrated to induce STAT2 phosphorylation.
Product Type Recombinant Protein, Carrier-Free
Specific Reactivity Human
Conjugate Unconjugated
Cross Reactivity Hu
Species E. coli
Recombinant Recombinant
Form Lyophilized
Protein Subtype Recombinant
Purity or Quality Grade Affinity chromatography
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