Invitrogen™ IFN gamma Human ELISA Kit, High Sensitivity

Product Code. 15551767

Brand: Invitrogen™ BMS228HS

Description

Includes

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-gamma
• Biotin-Conjugate anti-human IFN-gamma monoclonal antibody
• Streptavidin-HRP
• Human IFN-gamma Standard lyophilized, 200ng/mL upon reconstitution
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Amplification Diluent Concentrate (2x)
• Amplification Reagent I
• Amplification Reagent II
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution
• Stop Solution (1M Phosphoric acid)
• Adhesive Films

High Sensitivity Sandwich ELISA Kit

The Human High Sensitivity Interferon gamma (Hu HS IFNγ) ELISA quantitates Hu HS IFNγ in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IFNγ. Principle of the method The Human HS IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01579
• Assay Range: 0.16-10.0 pg/mL
• Assay Sensitivity: 0.06 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3458
• Gene Symbol: IFG,IFI
• Interassay CV: 7.1%
• Intraassay CV: 6.8%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Immune interferon, IFG
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 4 hr.

For Research Use Only