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  5. STEMCELL Technologies Human IFN-gamma ELISA Kit

STEMCELL Technologies Human IFN-gamma ELISA Kit

Product Code. 17108011
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Product Code. 17108011 Supplier STEMCELL Technologies Supplier No. 02002

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Description

For detection and measurement of human interferon-gamma

  • The Human Interferon-gamma (IFN-γ) ELISA Kit is designed for the quantitative detection and measurement of human IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants
  • IFN-γ is primarily secreted by T and NK cells
  • IFN-γ is a pleiotropic cytokine that modulates a broad range of immunological functions including antimicrobial and antitumor activity, and increases the surface expression of MHC class I and II proteins
  • The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine
  • The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody
  • Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample

Specifications

Description Dendritic Cells; Macrophages; NK Cells; T Cells
Quantity 2 Plates
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