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Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit

Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.

Brand:  Invitrogen™ 411102

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10177923

  • £2570.00 / 1 set


Estimated Shipment: 08-11-2021
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Description

Description

Assay IFN-a (human interferon alpha) levels in human serum, plasma and tissue culture media with these Thermo Scientific™ Human IFN alpha ELISA Kits (for Serum Samples).

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related,species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans,the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities),and single beta,omega,epsilon and kappa isoforms. Homologous molecules are found in many species,including rats and mice (and most mammals),and have been identified in birds,reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells,is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
Specifications

Specifications

HRP
HRP
Human IFN alpha ELISA Kit (Serum Samples)
Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
RUO
No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
5 x 96 microplate wells
3 hr. 15 min.
<8%
5 x 96 Tests
Supernatant
Colorimetric ELISA (TMB substrate) in clear 96-well plate
ELISA Kit
12.5-1000pg/mL
Plate Sealers, 20 ea, TMB Substrate Solution, 60mL, Concentrate Diluent, 60mL, Sample Diluent, 150mL, Antibody Concentrate, 1 vial, Dilution Buffer, 150mL, HRP Conjugate Concentrate, 1 vial, Human IFN Alpha Standard 10,000pg/mL, 2.5mL, Precoated 96-well Plates, 5 ea, Stop Solution, 60mL, Wash Solution Concentrate, 250mL
Absorbance at 450nm
Supernatant, 100 μL
2°C to 8°C
1 hr. 20 min.
Human
<8%
Human
IFNA1,IFNA2
Colorimetric Microplate Reader
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