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Invitrogen™ Human IFN-alpha 2 (alpha 2b) Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ 111051

Code : PN

Additional Details : Weight : 0.10000kg

Product Code. 10005793

  • £235.00 / Each
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Description

Description

Human IFN Alpha A (alpha 2b) Recombinant Protein, 111051, from Invitrogen., 111051, from Invitrogen. Species Reactivity: Human; Applications: ELISA, Immunoprecipitation, Western Blot.

The protein encoded by this gene is produced by macrophages and has antiviral activity. Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alpha-A) provided by the National Institutes of Health [see Pestka, S. (1986). Methods in Enzymology, Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV). Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum.
Specifications

Specifications

P01563
PBS with 0.1% BSA and no preservative
Human IFN alpha 2b
E. Coli
RUO
Human IFN alpha 2b
Human
Hu
Recombinant
Recombinant
ELISA, Immunoprecipitation, Western Blot
3440
1x105units
-80°C, Avoid Freeze/Thaw Cycles
Interferon alpha 2, IFNA2
IFNA2
Unconjugated
E. coli
Liquid
Ion-exchange chromatography, Size-exclusion chromatography
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