Invitrogen™ Human ID3 Control Fragment Recombinant Protein

Product Code. 30210601

Brand: Invitrogen™ RP94113

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-55152 (PA5-55152. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Specifications

• Accession Number: Q02535
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 3399
• Name: Human ID3 Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 1R21; bHLHb25; class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; HEIR-1; Helix-loop-helix protein HEIR-1; Hlh462; HLH642; ID 3; Id3; Id-3; Idb3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of DNA binding 3, HLH protein
• Common Name: ID3
• Gene Symbol: Id3
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: VPRGTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELAPELVISND
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining