Invitrogen™ Human Heparan Sulfate ELISA Kit

Product Code. 30245000

Brand: Invitrogen™ EEL199

Description

ELISA

Heparan sulfate (HS) is a glycosaminoglycan that is ubiquitously expressed on cell surfaces and in the extracellular matrix and basement membrane. Each HS molecule is a linear polysaccharide composed of repeating disaccharides of hexuronic acid and d-glucosamine that can exhibit immense structural diversity due to substitution to varying extents with sulfate groups and epimerization of glucuronic acid to iduronic acid, with areas of high sulfation and glucuronic acid epimerization being co-located in hot spots throughout the molecule. HS is structurally related to heparin, an extremely highly sulfated form of HS that is restricted to mast cells. The biosynthesis and modification of HS chains is thought to take place within the endoplasmic reticulum, Golgi apparatus, and trans Golgi network, which in the end produce unique HS chains that are covalently attached to a range of core proteins to form HS-proteoglycans [PMID: 24391644].

Specifications

• Assay Range: 0.31 to 20 ng/mL
• Assay Sensitivity: 0.19 ng/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: ELISA Microplate (Dismountable)
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <10
• Intraassay CV: <10
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: N-sulfated Heparan Sulfate
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.