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Invitrogen™ Human Hemoglobin ELISA Kit

Product Code. 17174523
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96 Tests
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17174523 96 Tests Pack of 96
17124999 5 x 96 Tests Each
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Product Code. 17174523 Supplier Invitrogen™ Supplier No. EH237RB

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ELISA

Human Hemoglobin quantitates human Hemoglobin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Hemoglobin.

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; delta globin; Haemoglobin; Hb; HBA1; HbA1c; HBA2; HBA-T3; HBH; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin subunit alpha; hemoglobin subunit alpha 1; hemoglobin, alpha 1
Assay Range 1.5 to 400 ng/mL
Assay Sensitivity 1.5 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3039, 3043
Gene Symbol HBA1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Hemoglobin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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