Invitrogen™ Human GTF2B Control Fragment Recombinant Protein

Product Code. 30203700

Brand: Invitrogen™ RP101317

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (99%), Rat (99%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84509 (PA5-84509. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Specifica

• Accession Number: Q00403
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 2959
• Name: Human GTF2B Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: general transcription factor 2 B L homeolog; general transcription factor IIB; general transcription factor TFIIB; GTF2B; gtf2b.L; gtf2b-a; gtf2b-b; LOW QUALITY PROTEIN: transcription initiation factor IIB; MGC137096 protein; RNA polymerase II alpha initiation factor; RNA polymerase II transcription factor IIB; RP11-82K18.1; S300-II; TF2B; tfiib; Transcription initiation factor IIB; Transcription initiation factor IIB-like protein; wu:fc44c11; XELAEV_18023356mg; XLTFIIB; zgc:66258
• Common Name: GTF2B
• Gene Symbol: GTF2B
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: LPRDTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFG
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining