Invitrogen™ Human GPR65 (aa 246-274) Control Fragment Recombinant Protein

Product Code. 30198881

Brand: Invitrogen™ RP109296

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (48%), Rat (48%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-140255 (PA5-140255. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

GPR65 also known as TDAG8 is a transmembrane protein belonging to the G protein coupled receptor super family with 337-amino acid and 90% similarity and 81% identity in the 7 transmembrane domains of its mouse homolog. It has 3 potential N-linked glycosylation sites and is expressed predominantly in lymphoid organs and cancer tissues with increased expression during activation-induced death of T-cell hybridomas. Two major transcripts of 4.5 kb and 1.8 kb are found in peripheral blood leukocytes and functions as a novel proton-sensing receptor. It couples to adenylyl cyclase and psychosine and is also actively involved in the establishment of immunological autotolerance. GPR65 has a role in cellular transformation by RhoA activation and actin rearrangement under conditions accompanying tissue acidosis.

Specifications

• Accession Number: Q8IYL9
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 8477
• Name: Human GPR65 (aa 246-274) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: Dig1; G protein-coupled receptor 65; Gpcr25; Gpr65; G-protein coupled receptor 25; G-protein coupled receptor 65; hTDAG8; Psychosine receptor; T cell death associated protein 8; T-cell death-associated gene 8 protein; TDAG8
• Common Name: GPR65
• Gene Symbol: GPR65
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: LLIRCILEHAVNFEDHSNSGKRTYTMYRI
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining