Gibco™ Human GPR15L Recombinant Protein, PeproTech®

Product Code. 17812883

Brand: Gibco™ 30071250UG

Description

Recombinant Protein

PeproTech's E.coli-derived Recombinant Human GPR15L consists of 57 amino acid residues and has a calculated molecular weight of 6.5 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

GPR15L is a newly identified ligand for GPR15, a member of the G protein-coupled receptor (GPCR) family. Upon ligation, GPR15L acts as a potent chemoattractant for GPR15-expressing T cells and together they mediate lymphocyte recruitment to the large intestine and skin. GPR15L is constitutively expressed by colon epithelial cells where its expression is minimally altered by intestinal inflammation. Conversely, GPR15L is nearly undetectable in adult epidermis but highly upregulated during wound healing and inflammation, particularly in psoriasis. Significant expression of GPR15L is also seen in additional mucosal epithelial cells, including those of the stomach, esophagus, and urinary tract. While maintaining similar expression patterns and intramolecular disulfide cysteine bridges found in members of the CC chemokine family, GPR15L differs from classic CC and CXC chemokines, whose active sites are found on the N-terminus, in that it relies on its C-terminus for receptor interaction. GPR15L was first identified as an antimicrobial peptide (AMP) due to its broad spectrum of antimicrobial activity, a property shared with many chemokines. GPR15L binds to an additional receptor, SUSD2, and early studies have indicated that both are downregulated in colon cancer tissue resulting in inhibited colon cancer growth.

Specifications

• Accession Number: Q6UWK7
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 387695
• Molecular Weight (g/mol): 6.5 kDa
• Name: Human GPR15L
• Quantity: 250 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Antimicrobial peptide with 57 amino acid residues; anti-microbial peptide with 57 amino acid residues; antimicrobial peptide-57; AP-57; C10orf99; chromosome 10 open reading frame 99; colon-derived SUSD2 binding factor; CSBF; GPR15L; Protein GPR15L; RLLV1833; secreted protein C10orf99; UNQ1833; UNQ1833/PRO3446
• Common Name: C10orf99
• Gene Symbol: C10orf99
• Biological Activity: Determined by its ability to chemoattract human PBMC's in a transwell-based migration assay.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: KRRPAKAWSG RRTRLCCHRV PSPNSTNLKG HHVRLCKPCK LEPEPRLWVV PGALPQV
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.