Invitrogen™ Human GPD2 (aa 547-664) Control Fragment Recombinant Protein

Product Code. 30209935

Brand: Invitrogen™ RP102982

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61022 (PA5-61022. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Mitochondrial glycerophosphate dehydrogenase (EC 1. 1. 99. 5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.

Specifications

• Accession Number: P43304
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 2820
• Name: Human GPD2 (aa 547-664) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: AA408484; AI448216; AU021455; AW494132; FAD-linked glycerol-3-phosphate dehydrogenase; GDH2; Gdm1; glycerol phosphate dehydrogenase 1, mitochondrial; glycerol phosphate dehydrogenase 2, mitochondrial; Glycerol-3-phosophate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase 2, mitochondrial; glycerol-3-phosphate dehydrogenase, mitochondrial; Gpd2; GPDH; GPDH-M; GPDM; GPD-M; mGPDH; mitochondrial glycerol-3-phosphate dehydrogenase; mitochondrial glycerophosphate dehydrogenase; mtGPD; mtGPDH; mtGPDH gene, promoter region and alternative transcripts; Protein TISP38; testicular tissue protein Li 76; TISP38
• Common Name: GPD2
• Gene Symbol: Gpd2
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: TAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLLPSDIDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTL
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining