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Globo H, clone: VK9, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9700-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15597606

  • £95.47 / 25µg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The VK9 monoclonal antibody recognizes human Globo H, a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.Applications Reported: This VK9 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.Applications Tested: This VK9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either high or low pH antigen retrieval at less than or equal to 5 ug/mL. The VK9 antibody has also been tested by western blot of reduced MCF-7 cell lysate and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Human Globo H
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
globoH, Globohexaosylceramide, Globo H antigen
IgG3, kappa
25μg
Store at 2-8°C.
Primary
Human
Flow CytometryþImmunohistochemistryþMicroscopyþWestern Blot
VK9
Unconjugated
Purified
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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