Gibco™ Human GDF-5, Animal-Free Recombinant Protein, PeproTech®

Product Code. 17806748

Brand: Gibco™ AF12001250UG

Beskrivning

Recombinant Protein

Recombinant Human GDF-5 (BMP-14/CDMP-1) is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Hunter-Thompson type dwarfism and in Grebe Syndrome, which is characterized by short stature, extra digits, and short and deformed extremities. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino acid signal peptide and a 354 amino acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.

Specifikationer

• Accession Number: P43026
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 8200
• Molecular Weight (g/mol): 27 kDa
• Name: Human GDF-5, Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 250 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: BDA1C; Bmp14; BMP-14; Bone morphogenetic protein 14; bp; brachypodism; brp; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP1; Cdmp-1; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; H-GDF-5; LAP4; LAP-4; Lipopolysaccharide-associated protein 4; LPS-associated protein 4; OS5; radotermin; SYM1B; SYNS2
• Common Name: GDF5
• Gene Symbol: GDF5
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 ug /ml.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: APLATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
• Content And Storage: -20°C
• Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 ug /ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.