Invitrogen™ Human FOLR3 ELISA Kit

Product Code. 17134959

Brand: Invitrogen™ EH201RBX5

Description

ELISA

Human FOLR3 quantitates human FOLR3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human FOLR3.

Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydrofolate into the cell. The folate receptors consist of five members, alpha, beta, gamma, gamma' (which is produced by alternative splicing) and delta. beta-FR is attached to the membrane by a GPI anchor and is expressed in malignant tissues of epithelial and nonepithelial origin. gamma-FR is expressed in tissues of hematopoietic origin, such as spleen, thymus and bone marrow. The expression pattern of delta-FR is elusive, which suggests that it is highly restricted both spatially and temporally. The alpha isoform of the folate receptor is used as a highly selective tumor marker and may be targeted for the delivery of therapeutic compounds to tumor cells by coupling to derivatives of folic acid.

Specifications

• Accession Number: Folate receptor 3; folate receptor 3 (gamma); folate receptor gamma; FOLR3; FR gamma; FR- gamma; FR-G; FR-gamma; gamma-hFR
• Assay Range: 32 to 8,000 pg/mL
• Assay Sensitivity: 32 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2352
• Gene Symbol: FOLR3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: FOLR3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins