Gibco™ Human FGFR1a (IIIc) Fc Recombinant Protein, PeproTech®

Product Code. 17870243

Brand: Gibco™ 16002500UG

Description

Recombinant Protein

Recombinant Human FGFR1a (IIIc) is a 65.2 kDa protein containing 586 amino acids. Under reducing conditions, FGFR1a migrates between 100-110 kDa on SDS-PAGE gel. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The FGF family plays a central role during prenatal development and postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. The FGF ligands bind to a family of type I transmembrane tyrosine kinase receptors, which leads to dimerization and activation by sequential autophosphorylation of specific tyrosine residues. Four genes encoding structurally related FGF receptors (FGFR-1 to -4) are known. Alternative splicing of the mRNAs generates numerous forms of FGFR-1 to -3. Alternate forms of FGF receptors can exhibit different specificities with respect to ligand binding. For example, the form designated as FGFR1a (IIc) interacts predominantly with FGF-acidic (FGF1) and FGF-basic (FGF2). A frequent splicing event involving FGFR-1 and -2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as thebeta isoform. Only the alpha isoform has been identified for FGFR-3 and FGFR-4. Additional splicing events for FGFR-1 to -3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc).

Specifications

• Accession Number: P11362
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 2260
• Molecular Weight (g/mol): 65.2 kDa
• Name: Human FGFR1a (IIIc) Fc
• Quantity: 500 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Common Name: FGFR1a (IIIc)
• Gene Symbol: FGFR1
• Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is = 0.1 ng/ml, corresponding to a specific activity of = 1 x 10^7 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: RPSPTLPEQA QPWGAPVEVE SFLVHPGDLL QLRCRLRDDV QSINWLRDGV QLAESNRTRI TGEEVEVQDS VPADSGLYAC VTSSPSGSDT TYFSVNVSDA LPSSEDDDDD DDSSSEEKET DNTKPNPVAP YWTSPEKMEK KLHAVPAAKT VKFKCPSSGT PNPTLRWLKN SKEFKPDHRI GGYKVRYATW SIIMDSVVPS DKGNYTCIVE NEYGSINHTY QLDVVERSPH RPILQAGLPA NKTVALGSNV EFMCKVYSDP QPHIQWLKHI EVNGSKIGPD NLPYVQILKT AGVNTTDKEM EVLHLRNVSF EDAGEYTCLA GNSIGLSHHS AWLTVLEALE ERPAVMTSPL YLEGGPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.