Invitrogen™ Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein

Product Code.	Quantity	unitSize
17124203	50 μg	50µg
17114203	5 μg	5µg

Product Code. 17124203 Supplier Invitrogen™ Supplier No. A42529

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Specifications

• Accession Number: P12318
• For Use With (Application): Control, Binding Assay, Immunohistochemistry, Surface Plasmon Resonance, Western Blot
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 2212
• Molecular Weight (g/mol): 22 kDa
• Name: Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 50 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: CD16; CD32; CD32A; CDw32; Fc fragment of IgG low affinity IIa receptor for (CD32); Fc fragment of IgG receptor IIa; Fc fragment of IgG, low affinity IIa, receptor; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor IIa; Fc receptor, IgG, low affinity III; FCG2; fc-gamma RII-a; Fc-gamma RIII; fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; Fcgr3; Fcgr3a; fcRII-a; fcRIII; FGCR; IGFR2; IgG Fc receptor II-a; igG Fc receptor III; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a; Low affinity immunoglobulin gamma Fc region receptor III
• Common Name: CD32a (FCGR2A)
• Gene Symbol: FCGR2A
• Biological Activity: 1.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 2.3 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested). 2.Loaded Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.65 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 3.Captured Bevacizumab (IgG1) on proA Chip can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 0.79 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 23-32 kDa verified by SEC-MALS.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human FCGR2A, amino acids Met1-Ile218 (Accession # P12318-1) with natural variant H167R substitution with a C-terminal His-tag
• Content And Storage: Store at -20 to -70 °C
• Activity: 1.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 2.3 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested). 2.Loaded Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.65 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested). 3.Captured Bevacizumab (IgG1) on proA Chip can bind Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein with an affinity constant of 0.79 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 4.The purity of Human Fc gamma RIIA (CD32a) (167 Arg/His) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 23-32 kDa verified by SEC-MALS.
• Endotoxin Level: < 1 EU/μg
• Expression System: HEK293 cells
• Protein Family: CD Proteins
• Protein Form: Cell Surface Protein, Recombinant, Truncated
• Form: Lyophilized
• Protein Subtype: Fc Receptors, Glycoproteins
• Research Category: Immunology, Signal Transduction
• Purity or Quality Grade: >95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC
• Protein: Fc fragment of IgG, low affinity IIa, receptor (CD32)