Invitrogen™ Endoglin (CD105) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHENG

Product Code. 15404983

Description

The Human Endoglin ELISA quantitates Hu Endoglin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu endoglin. Principle of the method The Human Endoglin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD105 (Endoglin is a Type I transmembrane protein) is highly expressed on human vascular endothelial cells and exists on an O- and N-glycosylated homodimer. Up regulation of CD105 expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumors and its gene expression is stimulated by hypoxia. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2. Mutations in the CD105 gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms of CD105 have been found. Diseases associated with CD105 dysfunction include hereditary hemorrhagic telangiectasia.

Specifications

• Accession Number: P17813
• Assay Sensitivity: 10 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 2022
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 12.35-3000 pg/mL
• Calibration Range: 10pg/mL to 3000pg/mL
• Conjugate: Biotin
• Protein Family: artery morphogenesis; BMP signaling pathway; bone development; cell adhesion; cell chemotaxis; cell migration; cell migration involved in endocardial cushion formation; cell motility; cellular response to mechanical stimulus; central nervous system vasculogenesis; chronological cell aging; detection of hypoxia; extracellular matrix constituent secretion; extracellular matrix disassembly; heart looping; intracellular signal transduction; negative regulation of cell migration; negative regulation of endothelial cell proliferation; negative regulation of nitric-oxide synthase activity; negative regulation of pathway-restricted SMAD protein phosphorylation; negative regulation of protein autophosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of collagen biosynthetic process; posi
• Specificity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: END,HHT1,ORW1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 5 μL; Serum, 5 μL; Supernatant, 50 μL
• Synonym: RP11-228B15.2, END, HHT1, ORW1
• Test Time: 1 hr. 20 min.