Invitrogen™ Human eIF3f (aa 104-174) Control Fragment Recombinant Protein

Product Code. 30206841

Brand: Invitrogen™ RP100070

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61918 (PA5-61918. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Specifications

• Accession Number: O00303
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 8665
• Name: Human eIF3f (aa 104-174) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 0610037M02Rik; AA409853; deubiquitinating enzyme eIF3f; eIF3 p47; eIF-3-epsilon; eIF3-epsilon; EIF3F; eIF3-p47; Eif3s5; eukaryotic translation initiation factor 3 subunit 5; eukaryotic translation initiation factor 3 subunit 5 epsilon; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon); eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47 kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47 kDa; eukaryotic translation initiation factor 3, subunit F
• Common Name: eIF3f
• Gene Symbol: EIF3F
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: DSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYA
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining