Invitrogen™ Human DNAJB2 (aa 73-168) Control Fragment Recombinant Protein

Product Code. 30204254

Brand: Invitrogen™ RP96083

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (85%), Rat (85%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-57590 (PA5-57590. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of the J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. Two isoforms are produced due to alternative splicing.

Specifications

• Accession Number: P25686
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 3300
• Name: Human DNAJB2 (aa 73-168) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 2700059H22Rik; CMT2T; DnaJ (Hsp40) homolog, subfamily B, member 10; DnaJ (Hsp40) homolog, subfamily B, member 2; DnaJ heat shock protein family (Hsp40) member B2; dnaJ homolog subfamily B member 10; DnaJ homolog subfamily B member 2; dnaJ protein homolog 1; Dnajb10; DNAJB2; DSMA5; Heat shock 40 kDa protein 3; heat shock protein J1; heat shock protein, DNAJ-like 1; heat shock protein, neuronal DNAJ-like 1; HSJ1; HSJ-1; HSPF3; mDj8
• Common Name: DNAJB2
• Gene Symbol: DNAJB2
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: LTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining