Invitrogen™ Human DLL4 Fc Chimera Recombinant Protein

Product Code. 17194183

Brand: Invitrogen™ A42512

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

Specifications

• Accession Number: Q9NR61
• For Use With (Application): Control, ELISA Standard, Binding Assay, Western Blot
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 54567
• Molecular Weight (g/mol): 81 kDa
• Name: Human DLL4 Fc Chimera
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 5 μg
• Source: HEK293 cells
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AOS6; delta 4; delta ligand 4; delta like canonical Notch ligand 4; Delta4; delta-like 4 (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4; UNQ1895/PRO4341
• Common Name: DLL4
• Gene Symbol: Dll4
• Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His (at 2 μg/mL (100 μL/well) can bind Human DLL4 Fc Chimera Recombinant Protein, the EC50 of human DLL4-hFc is 15-90 ng/mL 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 μg/mL in the presence of 500 ng/mL recombinant human BMP2.)
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Human DLL4, amino acids Met1-Pro524 (Accession # NP_061947.1) with a C-terminal human IgG1 Fc-tag
• Content And Storage: Includes 1 vial of lyophilized protein. Store at -20 °C.
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His at 2 μg/mL (100 μL/well) can bind Human DLL4 Fc Chimera Recombinant Protein, the EC50 of human DLL4-hFc is 15-90 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 μg/mL in the presence of 500 ng/mL recombinant human BMP2.
• Endotoxin Level: < 1 EU/μg
• Expression System: HEK293 cells
• Protein Family: Other Proteins
• Form: Lyophilized
• Protein Subtype: Neurotrophic Factors
• Research Category: Angiogenesis, Drug Development, Oncology, Signal Transduction
• Purity or Quality Grade: ≥95 % as determined by SDS-PAGE. ≥85 % as determined by SEC-HPLC.
• Protein: delta-like 4 (Drosophila)