Gibco™ Human DKK-3 Recombinant Protein, PeproTech®

Product Code. 17890793

Brand: Gibco™ 12046100UG

Description

Recombinant Protein

Recombinant Human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues. Due to glycosylation, Human DKK-3 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Specifications

• Accession Number: Q9UBP4
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 27122
• Molecular Weight (g/mol): 36.3 kDa
• Name: Human DKK-3
• Quantity: 100 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: AW061014; C87148; Dickkopf; dickkopf 3; dickkopf 3 homolog; Dickkopf gene 3; dickkopf homolog 3; dickkopf homolog 3 (Xenopus laevis); dickkopf WNT signaling pathway inhibitor 3; Dickkopf3; dickkopf-3; Dickkopf-related protein 3; DKK 3; Dkk3; dkk-3; hDkk 3; hDkk-3; hhDkk3; mDkk-3; p29; regulated in glioma; Reic; RIG; RIG-like 5-6; RIG-like 7-1; UNQ258/PRO295; Xenopμs
• Common Name: DKK3
• Gene Symbol: DKK3
• Biological Activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0-4.0 ng/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.