Gibco™ Human DKK-1 Recombinant Protein, PeproTech®

Product Code. 17870393

Brand: Gibco™ 12030500UG

Description

Recombinant Protein

Mature human DKK-1 expressed in HEK293 cells is a 35-40 kDa glycoprotein containing 235 amino acid residues. The calculated molecular weight of Recombinant Human DKK-1 expressed in HEK293 cells is 25.8 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head-forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play important regulatory roles in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. It has been suggested that by inhibiting Wnt/beta-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis.

Specifications

• Accession Number: O94907
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 22943
• Molecular Weight (g/mol): 25.8 kDa
• Name: Human DKK-1
• Quantity: 500 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: dickkopf 1 homolog; dickkopf homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf1; dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; dickkopf-related protein 1; DKK1; DKK-1; hDkk1; hDkk-1; mdkk-1; SK; UNQ492/PRO1008
• Common Name: DKK1
• Gene Symbol: DKK1
• Biological Activity: Determined by its ability to inhibit mWnt3a-induced TCF/LEF-luciferase activity in reporter HEK293 cells.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH
• Content And Storage: -20°C
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 97% by SDS-PAGE gel and HPLC analyses.