Invitrogen™ D-Dimer Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHDDIMER

Product Code. 15414973

Description

The Human D-Dimer ELISA quantitates Hu D-Dimer in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu D-Dimer. Principle of the method The Human D-Dimer solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibrinogen is the main protein of blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results in the activation of fibrinogen into fibrin by thrombin and fibrin polymerization. Fibrin clot is then digested by plasmin, and fibrin degradation products of different molecular weights are released into the bloodstream.

Specifications

• Accession Number: P02671
• Assay Sensitivity: 0.08 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 2243
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.082-60 pg/mL
• Calibration Range: 0.08pg/mL to 60pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This ELISA pair antibody detects human D-Dimer. Other species not determined.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Fib2
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
• Synonym: Fib2
• Test Time: 1 hr. 20 min.