Invitrogen™ Human Cyclophilin A Recombinant Protein

Product Code. 11554920

Brand: Invitrogen™ RP020

Description

Recombinant Protein

RP-020 can be used to catalyze the cis-trans-isomerization of X-Pro-peptide bonds. Protein folding reactions that are limited by the isomerization of X-Pro-peptide bonds are accelerated by cyclophilin A. RP-020 is a full-length human recombinant Cyclophilin A protein expressed in E. coli. Caution: Loss of protein may occur at high dilutions due to adsorption to container surfaces. Freezing of the enzyme or storage of the enzyme in diluted form could lead to loss of activity. In order to best maintain the endotoxin specification and peptidyl prolyl isomerase activity, it is recommended that aliquots be withdrawn with sterile pipet tips, if possible under a sterile hood.

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

Specifications

• Accession Number: P62937
• Concentration: 0.8 mg/mL
• For Use With (Application): Control, Binding Assay
• Formulation: 20mM tris HCl with no preservative; pH 7.8
• Gene ID (Entrez): 5478
• Molecular Weight (g/mol): 18 kDa
• Name: Human Cyclophilin A
• pH Range: 7.8
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. coli
• Storage Requirements: 4°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/μg
• Gene Alias: 2700098C05; Cphn; CYCA; cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; CyP-18; CYPA; CyP-A; CYPH; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; HGNC:9253; I79_011869; MGC117158; MGC12404; MGC23397; p1B15; p31; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase; PPIase A; rotamase; rotamase A; SP18; T cell cyclophilin
• Common Name: Cyclophilin A
• Gene Symbol: Ppia
• Biological Activity: Catalyzes the cis-trans-isomeration of X-Pro-peptide bonds. The protein folding that is limited by the isomeration of X-Pro-peptide bonds can be accelerated by cyclophilin A. Also binds to cyclosporins and the cyclophilin A/cyclosporin A.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Full-length
• Content And Storage: 4°C
• Activity: Catalyzes the cis-trans-isomeration of X-Pro-peptide bonds. The protein folding that is limited by the isomeration of X-Pro-peptide bonds can be accelerated by cyclophilin A. Also binds to cyclosporins and the cyclophilin A/cyclosporin A.
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE