Invitrogen™ Human Coagulation Factor XIV/Protein C ELISA Kit

Product Code. 17114869

Brand: Invitrogen™ EH119RBX5

Description

ELISA

Human Coagulation Factor XIV/Protein C quantitates human Coagulation Factor XIV/Protein C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Coagulation Factor XIV/Protein C.

Protein C is a vitamin K-dependent plasma glycoprotein that is cleaved to its activated form by the thrombin-thrombomodulin complex. Protein C in its activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 μg/mL. Mutations in the Protein C gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Specifications

• Accession Number: Activation peptide; anticoagulant protein C; APC; Autoprothrombin IIA; blood coagulation factor XIV; EDQVDPRLIDGK; inactivator of coagulation factors Va, VIII; PC; prepro-protein C; Proc; PROC1; protein C; protein C (inactivator of coagulation factors Va and VIIIa); protein C prepropeptide; protein C, inactivator of coagulation factors Va and VIIIa; THPH3; THPH4; type I protein C; vitamin K-dependent protein C; Vitamin K-dependent protein C heavy chain; Vitamin K-dependent protein C light chain
• Assay Range: 0.82 to 20 ng/mL
• Assay Sensitivity: 0.82 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5624
• Gene Symbol: PROC
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Protein C
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins