Gibco™ Human CNTF Recombinant Protein

Product Code. 10583395

Brand: Gibco™ PHC7015

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute Ciliary Neurotrophic Factor in 5-10 mM sodium phosphate, pH 7.5, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.

Specifications

• Accession Number: P26441
• For Use With (Application): Bioactivity
• Formulation: 5mM sodium phosphate with 75mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 1270
• Molecular Weight (g/mol): 22.7 kDa
• Name: Human CNTF
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 20 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: AI429687; Ciliary neurotrophic factor; ciliary neurotropic factor; Cntf; HCNTF; H-CNTF
• Common Name: CNTF
• Gene Symbol: CNTF
• Biological Activity: ED50 = 50 - 150 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human CNTF recombinant protein contains 199 amino acids
• Content And Storage: -20°C
• Activity: ED50 = 50 - 150 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Neurotrophic Factors
• Research Category: Stem Cell Research, Differentiation
• Product Line: Gibco
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC