Invitrogen™ Human CHCHD6 (aa 2-71) Control Fragment Recombinant Protein

Product Code. 30211915

Brand: Invitrogen™ RP100169

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (47%), Rat (47%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61567 (PA5-61567. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

CHCHD6 is a member of a family of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain and has been observed in a complex with the mitochondrial proteins mitofilin, SAM50, metaxins 1 and 2, and CHCHD3. CHCHD6 knockdown causes severe defects in mitochondrial cristae morphology as well as reductions in cell growth, ATP production and oxygen consumption. This decrease of CHCHD6 also led to a reduction in mitofilin protein levels, while a knockdown of mitofilin resulted in a reduced level of CHCHD6, suggesting coordinate regulation between these two proteins. CHCHD6 knockdown in human cancer cells enhances their sensitivity to genotoxic anticancer drugs, suggesting that CHCHD6 may be a potential therapeutic anti-tumor target.

Specifications

• Accession Number: Q9BRQ6
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 84303
• Name: Human CHCHD6 (aa 2-71) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 0710001P09Rik; 1700021B03Rik; CHCHD6; CHCM1; coiled coil helix cristae morphology 1; coiled coil helix cristae morphology protein 1; coiled-coil-helix cristae morphology 1; coiled-coil-helix cristae morphology protein 1; coiled-coil-helix-coiled-coil-helix domain containing 6; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6; coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial; Mic25; MICOS complex subunit MIC25; PPP1R23; protein phosphatase 1, regulatory subunit 23
• Common Name: CHCHD6
• Gene Symbol: CHCHD6
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: GSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPR
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining