Invitrogen™ Human BAFF (BLyS) Recombinant Protein

Product Code. 15517556

Brand: Invitrogen™ 14894380

Description

Recombinant Protein

Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity. Applications Reported: Recombinant human BAFF is biologically active. Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6 μg/mL. Source: E. coli expressed amino acids Ala134-Leu285 accession # Q9Y275. Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6 μg/mL. Endotoxin: Less than 0.40 ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE. Molecular Weight: 18.5 kDa. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE.

BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

Specifications

• Accession Number: Q9Y275
• Concentration: 0.1 mg/mL
• For Use With (Application): Bioactivity, Functional Assay
• Formulation: PBS with no preservative
• Gene ID (Entrez): 10673
• Molecular Weight (g/mol): 18.5 kDa
• Name: Human BAFF (BLyS)
• Purification Method: Purified
• Quantity: 25 μg
• Source: E. coli
• Storage Requirements: -80°C
• Regulatory Status: RUO
• Endotoxin Concentration: <0.40 ng/μg
• Gene Alias: ApoL related ligand TALL-1; b cell-activating factor; B lymphocyte stimulator; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; D8Ertd387e; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; RGD1561519; TALL1; TALL-1; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; Tnfsf13b; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; UNQ401/PRO738; ZTNF4
• Common Name: BAFF (BLyS)
• Gene Symbol: TNFSF13B
• Biological Activity: ED50 = 1.6 μg/mL; determined by a mouse splenocyte survival assay.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human BAFF, amino acids Ala134-Leu285 (Accession # Q9Y275)
• Content And Storage: -80°C
• Activity: ED50 = 1.6 μg/mL; determined by a mouse splenocyte survival assay.
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >90% by reducing SDS-PAGE