Invitrogen™ Human CD229 ELISA Kit

Product Code. 17164833

Brand: Invitrogen™ EH82RB

Description

ELISA

Human CD229 quantitates human CD229 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD229.

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

Specifications

• Accession Number: AI893573; CD229; CDABP0070; Cell surface molecule Ly-9; hly9; Lgp100; Ly9; Ly-9; lymphocyte antigen 9; mLY9; RP11-312J18.1; Signaling lymphocytic activation molecule 3; SLAM family member 3; SLAMF3; T100; T-lymphocyte surface antigen Ly-9
• Assay Range: 80 to 20,000 pg/mL
• Assay Sensitivity: 80 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4063
• Gene Symbol: Ly9
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD229 (Ly9)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins