Gibco™ Human CD14 (soluble) Recombinant Protein, PeproTech®

Product Code. 17800703

Brand: Gibco™ 1100150UG

Description

Recombinant Protein

Recombinant sCD14 is a 331 amino acid glycoprotein comprising the extracellular portion of the CD14 receptor. The calculated molecular weight of Recombinant Human sCD14 is 35.6 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Specifications

• Accession Number: P08571
• For Use With (Application): ELISA, Functional Assay, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 929
• Molecular Weight (g/mol): 35.6 kDa
• Name: Human CD14 (soluble)
• Quantity: 50 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
• Common Name: CD14
• Gene Symbol: Cd14
• Biological Activity: Determined by the dose-dependent activation of NF-kappaB in a RAW264 cell line based reporter system, using a sCD14 concentration range of 20 ng/ul to 200 ng/ul The NF-kappaB activation is enhanced when the assay is done in the presence of 0.25 ng/ul to 1.0 ng/ul bacterial LPS.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P
• Content And Storage: -20°C
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.