Invitrogen™ Human CCBE1 (aa 159-250) Control Fragment Recombinant Protein

Product Code. 30181817

Brand: Invitrogen™ RP98285

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (89%), Rat (89%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59534 (PA5-59534. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The gene encoding CCBE1 (Collagen and calcium-binding EGF domain-containing protein 1) is located on chromosome 18 in a region that is frequently found to be deleted in breast and prostate cancers. CCBE1 is a 406 amino acid secreted protein that contains two collagen-like domains and one calcium-binding epidermal growth factor (EGF-like) domain. In general, EGF-like domains include six cysteine residues that are usually involved in disulfide bonds. Calcium-binding EGF-like domains are subject to amino acid substitutions that disrupt their structure or calcium affinity, therefore altering protein function. Downregulation of the gene encoding CCBE1 is observed with high frequency in breast cancer, suggesting that loss of CCBE1 results in changes in cell adhesion and mobility due to its characterization as an extracellular protein. There are three named isoforms of CCBE1 which are produced as a result of alternative splicing events.

Specifications

• Accession Number: Q6UXH8
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 147372
• Name: Human CCBE1 (aa 159-250) Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 4933426F18Rik; 9430093N24Rik; CCBE1; collagen and calcium binding EGF domains 1; collagen and calcium-binding EGF domain-containing protein 1; FLJ30681; fof; full of fluid protein; Full of fluid protein homolog; HKLLS1; KIAA1983; MGC50861; mKIAA1983; RGD1307670; UNQ1921/PRO4395
• Common Name: CCBE1
• Gene Symbol: CCBE1
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: CECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPG
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining