Invitrogen™ Human Complement C5a ELISA Kit

Product Code. 15314988

Brand: Invitrogen™ BMS2088

Description

ELISA

The Human Complement C5a (Hu C5a) ELISA quantitates Hu C5a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C5a. Principle of the method The Human C5a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.

Specifications

• Accession Number: P01031
• Assay Range: 0.078 to 5.0 ng/mL
• Assay Sensitivity: 0.005 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 727
• Gene Symbol: C5Da,C5b,CPAMD4,ECLZB,C5
• Interassay CV: 4%
• Intraassay CV: 3.6
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 5 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: complement component 5, C5a, C5D, C5b, ECLZB, CPAMD4
• Target: Complement C5a
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 30 mins