Gibco™ Human BMP-4 Recombinant Protein

Product Code. 11330852

Brand: Gibco™ PHC9534

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human BMP-4 in 4 mM HCl containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store this lyophilized preparation at =-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at =-20°C. Avoid repeated freeze/thaw cycles.

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

Specifications

• Accession Number: P12644, P21275
• For Use With (Application): Bioactivity
• Formulation: HCl with no preservative
• Gene ID (Entrez): 12159, 652
• Molecular Weight (g/mol): 34 kDa
• Name: Human BMP-4
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: BMP; BMP2B; BMP-2B; Bmp2b1; Bmp2b-1; Bmp4; Bmp-4; BOMPR4A; Bone morphogenetic protein; Bone morphogenetic protein 2B; bone morphogenetic protein 4; bone morphogenetic protein 4 preproprotein; DVR4; Dvr-4; MCOPS6; OFC11; ZYME
• Common Name: BMP4
• Gene Symbol: BMP4
• Biological Activity: ED50 = 2 - 10 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: ED50 = 2 - 10 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.
• Bioassay: Induction of alkaline phosphatase in ATDC5 cells
• Endotoxin Level: <1 EU/ml
• Shipping Condition: Dry Ice
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: BMPs (Bone Morphogenetic Proteins) & Related Factors
• Research Category: Bone Research, Stem Cell Research, Differentiation
• Product Line: Gibco
• Purity or Quality Grade: >95% by SDS-PAGE